The original focus of the BioLingus Technology was the oral administration of proteins, and more in particular cytokines.
A major challenge to the oral formulation of peptides and proteins into efficacious dosage forms is to ensure the stability over their shelf life. Most injectable proteins are marketed as lyophilized products, and most of these need refrigerated storage.
Typically, a shelf life of at least 2 years at room temperature is desirable. Most injectable proteins are marketed as lyophilized products, and most of these need refrigerated storage.
The BioLingus technology has been developed initially by improving the stability of the cytokines in an oral tablet. This resulted in what is called the "Cytokine Stabilization and Slow Release" technology, abbreviated as CSSR.
Over time, multiple "generations" of the technology have been developed, and the latest is called "CSSR-4" – being the fourth generation. The result can be seen in the graph below. The data demonstrate the CSSR-formulation of interferon-alfa is stable at 42 months at room temperature.
The technology behind the formulation stability is inspired by nature…
Although it is generally accepted in science that "proteins are not very stable", nature has developed a successful way to stabilize proteins over a very long time.
Figure : Nelumba nucifera (Indian Lotus) : in the seeds of the Indian lotus plant, proteins are stabilized and can preserve their functionality up to 1000 years.
The scientific mechanism that enables plant seeds to preserve the functionality of proteins over such a long time, many times in extreme conditions of temperature and moisture, has been studied over decades.
Through advanced "bio-engineering", the BioLingus SEED Technology is mimicking what is happening in nature in a plant SEED.
So the SEED technology is our specialized formulation to stabilize biological molecules.